Quantitative analysis of the cellular growth patterns shaping the Arabidopsis ovule

拟南芥胚珠细胞生长模式的定量分析

• 批准号: 357138058
• 负责人: Professor Dr. Kay Schneitz
• 金额: --
• 依托单位: Professur für Entwicklungsbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/357138058?language=en
• 关键词: Quantitative; analysis; cellular; growth; patterns

How an organ attains its three-dimensional architecture represents a salient question in biology. Plant organs are characterized by a huge diversity in sizes and forms. A striking example is provided by the strongly bent Arabidopsis ovule. Ovule curvature is the consequence of poorly understood cellular and tissue-level events that occur during growth of the two integuments, lateral sheet-like tissues that eventually develop into the seed coat. We use Arabidopsis integuments as model system to study how tissue sheets develop into complex three-dimensional shapes. In previous work we succeeded in generating 3D digital ovule models with cellular resolution encompassing all developmental stages. In this project we will build on and extend those efforts. We will focus on the question how the ovule bends itself to its final shape. To this end we will apply genetics, advanced imaging and mathematical modelling of growth. In an iterative approach between experiment and model-based predictions, we will investigate the cellular mechanism underlying integument growth and ovule curvature. This interdisciplinary approach will not only improve our understanding of ovule curvature but will also contribute to the general knowledge of how organs shape themselves into complex three-dimensional structures.

器官如何形成三维结构是生物学中的一个突出问题。植物器官的特点是大小和形式的巨大差异。强烈弯曲的拟南芥胚珠就是一个显著的例子。胚珠弯曲是在两个被珠生长过程中发生的细胞和组织水平事件的结果，两个被珠是最终发育成种皮的侧片状组织。我们使用拟南芥被作为模型系统来研究组织薄片如何发展成复杂的三维形状。在之前的工作中，我们成功地生成了包含所有发育阶段的细胞分辨率的3D数字胚珠模型。在这个项目中，我们将以这些努力为基础并加以扩展。我们将集中讨论胚珠如何弯曲成最终形状的问题。为此，我们将应用遗传学，先进的成像和数学模型的增长。在实验和基于模型的预测之间的迭代方法中，我们将研究被皮生长和胚珠弯曲的细胞机制。这种跨学科的方法不仅将提高我们对胚珠曲率的理解，而且还将有助于了解器官如何将自己塑造成复杂的三维结构。