Development of amyloid imaging using fluorine-MRI

本研究的目的是利用19 F MRI开发淀粉样蛋白成像。我们开发了一种新型的含19 F的姜黄素衍生物（命名为FMeC 1或Shiga-Y 5）作为淀粉样蛋白成像的潜在19 F MRI探针。当Tg 2576小鼠（阿尔茨海默病的转基因小鼠模型）在20-22月龄时外周注射FMeC 1时，在从整个头部获得的19 F MR谱中观察到19 F信号水平的剂量依赖性增加。在注射200 mg/kg剂量的FMeC 1后，19 F MR图像显示Tg 2576小鼠脑中显著水平的19 F信号。在注射FMeC 1的野生型小鼠的脑中没有显著的19 F信号。注射FMeC 1的Tg 2576小鼠脑的组织学分析显示FMeC 1的强荧光信号，并且该信号的大部分与β-淀粉样蛋白肽免疫反应性共定位。这些结果表明，19 F MRI与FMeC 1淀粉样蛋白成像的潜在好处。

The aim of this study is to develop amyloid imaging using 19F MRI. We have developed a novel 19F-containing curcumin derivative (named FMeC1 or Shiga-Y5) as a potential 19F MRI probe for amyloid imaging. When Tg2576 mice, a transgenic mouse model of Alzheimer's disease, at 20-22 months of age were peripherally injected with FMeC1, dose-dependent increasing in the level of 19F signal was observed in 19F MR spectra obtained from the whole head. 19F MR image showed remarkable levels of 19F signal in the brain of Tg2576 mice after the injection of FMeC1 at a dose of 200 mg/kg. There are no significant 19F signals in the brain of FMeC1-injected wild-type mice. Histological analysis of FMeC1-injected Tg2576 mouse brain showed strong fluorescence signal for FMeC1, and most of this signal was co-localized with beta-amyloid peptide-immunoreactivity. These results suggest the potential benefit of 19F MRI with FMeC1 for amyloid imaging.