Development of regenerative medicine using human iPS cellsFor pulp and periapical pathoses

在低氧条件下培养的诱导多能干细胞的生长和分化以及诱导iPS细胞向成骨细胞的分化方面，四种转录因子组与三种转录因子组之间没有统计学差异。此外，（缺氧诱导因子）HIF在小鼠iPS细胞在缺氧条件下的功能进行了研究与形态和转录因子的表达。HIF-2α敲低组iPS细胞集落大小减少。与对照组相比，HIF-2α或-3α组的转录因子表达出现统计学显著性降低。这些结果表明，HIF-2α是HIF中维持小鼠iPS细胞多能性的最有影响力的候选者。

There are no stastical differences in either the growth and differentiation of (induced pluripotent stem) iPS cells or the induction of iPS cells to osteoblasts between the four and three transcription factor froups cultured under hypoxia. Furthermore, the function of (hypoxia inducible factors) HIFs in murine iPS cells under hypoxia was investigated in relation to the morphology and expression of the transcription factors. The HIF-2α knockdown group exhibited a decreased colony size in the iPS cells. The HIF-2α or -3α demonstrated a statistical significant decrease in the expression of the transcription factors compared to those observed in the control group. These results demonstrate that HIF-2α among HIFs is the most influential candidate for the maintenance of the pluripotency of murine iPS cells.