Inhibitory effects of the simultaneous intake of zinc and vitaminsE and C on blood pressure elevation and vascular thickness

摄入饮食中的抗氧化剂可以减少活体内的氧化压力。饮食控制高血压(DASH)建议饮食控制血压(BP)。其作用机制可能与蔬菜和水果的抗氧化作用有关，但对其研究数据尚不明确。甚至最有可能影响血压的抗氧化剂维生素E和C也没有被证实能控制血压。由于许多抗氧化营养素相互加强或相互抵消，我们假设某些抗氧化营养素的组合可能会降低高血压患者的血压升高。为了验证这一假说，我们建立了雄性6周SD大鼠双肾一夹肾血管性高血压模型，在血压升高后喂饲含锌和维生素E和C的饲料，并观察血压和血管紧张素Ⅱ的产生。结果表明，单独摄入锌、维生素E、C对高血压模型大鼠血压无明显影响，但三种营养素合用可抑制血压升高，降低血浆血管紧张素Ⅱ水平。在大鼠中，血管壁厚度减少，血压升高受到抑制。我们还通过几种程序对血管进行染色，对其机制进行了研究。目前正在通过流式细胞仪和荧光显微镜观察中性粒细胞对内皮细胞的影响，从血管内部来研究这种影响。

Intake of dietary antioxidants reduces oxidative stress in a living body. Dietary Stop to Hypertension（DASH）proposed dietary control of blood pressure (BP) control. The mechanism of the control may involve the antioxidative effects of vegetable and fruits,but the data of the investigations on it are still equivocal. Even vitamins E and C, which were most probable antioxidants to affect BP, were not confirmed to control BP. Since many antioxidative nutrients reinforce or cancel each other, we hypothesized that some combination of antioxidative nutrients may reduce BP elevations in hypertension. To test the hypothesis, we induced 2-kidney, 1-clip renovascular hypertension into male 6 wk Sprague Dawley rats, fed diets containing zinc and vitamins E and C after BP elevation, and observed BP and angiotensin (Ang) II production. As a result, we observed that each sole intake of zinc or vitamin E or C did not affect BP, but the combination of the three nutrients inhibited the BP elevation and decreased plasma AngII level, in the hypertensive model rats. In the rats, the wall thickness of blood vessels was observed to be reduced with the inhibition of BP elevation. We also investigated the mechanism, by staining blood vessels with several procedures. It is now on going to investigate the effects from the inside of vessels by observing the effects of neutrophil on endothelial cells using flow cytometry and fluorescence microscopy.