Structural bases for an expanded codon recognition by modified nucleotide.

在tRNA反密码子的第一个字母上的一些修饰的核苷酸已知可以扩展密码子识别能力。我计划根据70S核糖体中密码子-反密码子碱基对的三维结构揭示其机制。在这项研究中，我成功地建立了一种高衍射晶体制备70S核糖体的可重复性方法。一旦获得了良好的晶体，就需要在以下步骤中进行技术改进：改进结晶条件，减少收获过程中晶体的损坏，以及在原子分辨率上收集数据的策略。

Some modified nucleotides in the first letter of a tRNA anticodon is known to expand the codon recognition ability. I planed to reveal the mechanism based on the three-dimensional structure of the codon-anticodon basepair in the 70S ribosome.In this study, I have succeeded to establish a reproducible method of 70S ribosome preparation for highly diffracted crystals. Once good crystals are obtained, technical improvements are required in the following steps: refinement of crystallization condition, less damage for crystals during harvesting, and strategy of data collection at an atomic resolution.