Analyses of influence by Warburg Effect small molecular Metbolites via Cancer-Stroma interactions

小分子代谢产物浓度的局部波动，如沃堡效应产生的乳酸或丙酮酸，经常出现在癌组织及其周围。在这项研究计划中，我们通过肿瘤-间质相互作用，研究这些代谢物浓度变化对肿瘤组织、维持和恶化的分子影响和独特表达的机制。利用由癌细胞和间质细胞组成的共培养细胞模型系统，我们利用新的生物信息学技术，从整合的DNA芯片表达数据中成功地阅读了每个细胞组的半独立表达谱。提取了几个基因产物，并证明这些表达是对这些代谢物浓度变化的特异性反应。分子信号转导途径是通过使用显示出类似分子结构的试剂来推测的，这些试剂具有小分子代谢物、抑制物和激活剂来实现Warburg效应。

Local fluctuations of small molecular metabolic products concentration, like lactate or pyruvate produced with Warburg effect, were frequently seen in and around cancer tissue. In this research plan, we investigate molecular influences and mechanism of unique expression due to these metabolites concentration changes, via cancer - stroma interaction, for organization, maintenance, and exacerbation of cancer.Using co-cultured cell model system composed with cancer cell and stroma cell, we successfully perused semi-independent expression profiles of each cell group from integrated DNA chip expression data with novel bio-informatics techniques. Several gene products were extracted, and certified as these expressions were specifically responded to those metabolite concentration changes. Molecular signal transduction pathways were presumed by experiments using reagents showing resembled molecular - structure with small molecular metabolites, inhibitors, and activators for Warburg effect.