Application of rapid gene analyzing method for susceptibility to cancer

已知MDM 2是一种与p53蛋白结合并使p53的肿瘤抑制活性失活的癌蛋白。MDM 2基因的遗传多态性被认为是肿瘤易感性标志物和癌症的预后因素。本研究采用智能扩增技术（Smart Amplification Process，SmartPCR）检测MDM 2基因中c.309T>G单核苷酸多态性（SNP），并进行了大规模病例对照研究。我们发现MDM 2 SNP 309基因型与肺癌风险之间没有显著关联。吸烟相关肺癌的亚组分析表明，SNP 309基因G/G基因型可能与肺癌的发生有关。

MDM2 is known as an oncoprotein that binds to p53 protein and inactivates the tumor suppressor activity of p53. Genetic polymorphisms in the MDM2 gene are suggested to be a tumor susceptibility marker and a prognostic factor for cancer. In this study, we have developed a new SNP detection method applied Smart Amplification Process (SmartAmp) method to detect the SNP c.309T>G in the MDM2 gene and conducted a large-scale case-controlled study. We found that there were no significant associations between MDM2 SNP309 genotypes and lung cancer risk. Subgroup analysis of smoking related lung cancer indicated that G/G genotype of SNP309 may be associated with lung cancer carcinogenesis.