Peculiar mechanisms of reperfusion injury and acute rejection after lung transplantation

WT小鼠同种异体移植物在移植后5天出现严重的急性排斥反应。在移植后5天，egr -1缺失的供体小鼠的肺移植只出现了最小的排斥反应。在第1天观察到Egr-1 mRNA转录物的强烈上调，然后在接下来的5天内下降。Egr-1靶介质（IL-1β、MCP-1和PAI-1）的mRNA在第5天达到最高水平。无egr -1的同种异体移植物il -1β和MCP-1 mRNA的表达明显降低（p< 0.05）。05)。我们的研究表明，在肺同种异体移植物中删除Egr-1可以通过减少化学介质的表达水平来改善严重的急性排斥反应，这意味着治疗急性肺同种异体移植物排斥反应的一种新的可能策略。

Severe acute rejection was observed in allografts from WT mice at 5 days after transplantation. Only minimal rejection was seen in the lung graft from Egr-1-null donor mice at 5 days after transplantation. Strong upregulation of Egr-1 mRNA transcripts was observed at day 1, which then decreased during the next 5 days. The mRNA of Egr-1 target mediators(IL-1β, MCP-1, and PAI-1) reached maximal levels at day 5. Egr-1-null allografts exhibited significantly lower expressions of IL-1βand MCP-1 mRNA(p<0. 05). Our study showed that deletion of Egr-1 in lung allografts ameliorates severe acute rejection with the reduction of expression levels of chemical mediators, implying a new possible strategy for treating acute pulmonary allograft rejection.