Identification of the gene coding tyrosine hydroxylase using the transposon-tagging methods.

从紫茉莉（Mirabilis jalapa）中分离出DOPA双加氧酶（DOD）基因的启动子区。DNA序列分析表明，DOD基因由3个外显子和2个内含子组成。然而，我们在基因组区域中没有发现任何转座元件样序列。采用cDNA差减法从紫茉莉花瓣中分离到3个酪氨酸羟化酶的候选cDNA。

The genomic region including promoter of the DOPA dioxygense(DOD) gene was isolated from Mirabilis jalapa. DNA sequence analysis revealed that the DOD gene was consisted of 3 exons and 2 introns. We, however, could not find any transposable element-like sequences in the genomic region. Three candidate cDNAs encoding tyrosine hydroxylase were isolated from petals of Mirabilis jalapa using cDNA subtraction method.