栄養素の過不足を利用したプリ骨格異常モデルの構築

荧光钙黄蛋白染色成功地显示了黄尾鱼在孵化后2天和14天的喙部、牙髓、鳃弓和神经头骨。钙黄蛋白染色也能观察到15 dph左右的神经脊柱和脊骨的其他部分。然而，鱼体表面的色素形成阻碍了鱼背部骨骼在18 dph左右的直接观察。此外，在22英里/小时左右，身体的增厚往往使鱼无法直接观察轴向骨骼，而在27英里/小时时则变得不可能。为了分析生长抑制与骨骼畸形发生的一致性，对同一批次不同大小的鱼进行了骨骼畸形发生的检查。在1^<st>实验中，体型越大的鱼，骨骼畸形的发生率就越高。然而，在明显的对比中，体型较小的鱼出现骨骼畸形的频率更高。因此，鱼类的生长速度与骨骼畸形的发生之间没有明确的关系。

Fluorescent calcein staining successfully visualized cleithrum, and dentary, gill arch and neurocranium of yellowtail at 2 and 14 days post hatching(dph), respectively. Calcein staining also visualized neural spine and other elements of back bone around 15 dph. However, pigment formation at the surface of fish body hinder direct observation of back bone of fish at around 18 dph. Moreover, thickening of body often prevented direct observation of axial skeleton in fish at around 22 dph and becomes impossible at 27 dph. To analyze coincidence of growth depression and occurrence of skeletal deformity, occurrence of bone deformity was examined in fish showing different sizes from same batch of fish. in the 1^<st> experiment, significantly higher incidence of skeletal defomrity was observed in fish from bigger size of fish. However, in apparent contrat, smaller size fish showed higher frequency of occurrence of skeletal deformity. Therefore, there was no clear relationship between growth rate and occurrence of skeletal deformity in fish.