Development of environmentally friendly pretreatment using ferric catalyst as abrasive for enzymatic hydrolysis of lignocellulosic biomass

为了实现柏木针叶木材的高效酶解，研究了干式盘磨与研磨剂和碱性过氧化氢处理相结合的方法，确定了它们的适宜条件。处理后的样品具有很高的酶消化率，这是因为这种组合导致了几乎一半的半纤维素和60%的木质素的降解，并使暴露的纤维素纳米纤维和纳米尺度在表面“隆起”。此外，还确定了合适的酶，降低了酶的负载量。

To achieve highly effective enzymatic hydrolysis of Japanese cypress (Chamaecyparis obtusa), softwood, the combination of dried disk milling with abrasives and alkaline-peroxide treatment was investigated, and their suitable conditions were decided. The treated sample had highly enzymatic digestibility because this combination caused the degradation of almost of hemicellulose and 60% of lignin, and the exposing cellulose nanofiber and nano scale " raising" on the surface. Moreover, the suitable enzyme was decided, and it was possible to reduce the amount of loading enzymes.