Analysis of input signal specific to mammalian circadian center

为了研究视交叉上核（SCN）的信号传递，我们对Per2:: Luc大鼠SCN片长期给予福斯克林（foskolin， FK）对cAMP信号的扰动。FK的管理将SCN根据周期长度分为两个区域。其中1个周期短于24 h(SPR)， 1个周期长于24 h（LPR）。SPR定位于SCN背侧内侧，LPR定位于外侧。有趣的是，SPR和相波起始区域是重叠的。这些结果表明cAMP依赖信号参与了SCN亚区的同步。对SPR的进一步研究可能有助于了解相波在哺乳动物昼夜节律中心中的未知机制和作用。

In order to investigate signals transmitted in the suprachiasmatic nucleus(SCN), perturbation of cAMP signal by chronic administration of Forskolin(FK) to Per2 :: Luc rat SCN slice was performed. Administration of FK divided the SCN into two regions based on the period length. One of which showed shorter period than 24 h(SPR) and another showed longer than 24 h(LPR). SPR was localized in the medial region of the dorsal SCN and LPR localized in the lateral region, respectively. Intriguingly, the SPR and a region where phase wave starts were overlapped. These results suggest that cAMP dependent signal is involved in the synchronization of the SCN subregions. Further study of SPR may lead to the understanding of unknown mechanisms and roles of the phase wave in the mammalian circadian center.