Challenge to oocyte quality improvement by introduction of embryonic gene

Oct3/4等胚胎基因在基因组启动中起作用。近年来，有研究表明胚胎基因在卵母细胞/胚胎发育中起着重要作用。我们考察了这些胚胎基因的引入是否会提高胚胎发育的能力。我们分别制作了Oct3/4、Sox2、Nanog的cRNA+GFP探针，并检测了它们在体内和体外的功能。此外，我们还评估了这些CRNAs导入后卵子的胚胎发育能力。发现导入CRNAs后胚胎发育能力有提高的趋势。然而，我们并没有得到关于老化个体卵母细胞发育改善的明确结论。

The embryonic genes such as Oct3/4 function for genomic initialization. Recently, it is suggested that embryonic genes function for the oocyte/embryonal development. We examined whether improvement of the ability for embryonic development by introduction of these embryonic genes. We made cRNA+GFP probe of the each Oct3/4, Sox2, Nanog and examined functions in in vivo and in vitro. Furthermore, we evaluated embryonal developmental ability of the egg after introduction of these cRNAs. The tendency that embryonic developmental ability improved after introduction of cRNAs, was found. However, we did not obtain clear conclusions about the developmental improvement of oocytes from an aging individual.