Identification of novel tyrosine kinase fusion genes using a unique system based on morphology

我们在炎性肌纤维母细胞肿瘤中鉴定了PPFIB 1-ALK，在肾细胞癌中鉴定了TPM 3-ALK和EML 4-ALK。在淋巴瘤中，我们发现了SQSTM 1-ALK。通过针对福尔马林固定石蜡包埋（FFPE）优化的RACE，从FFPE组织中鉴定出KLC 1-ALK。我们建立了一个独特的基于组织病理学方法的融合基因鉴定系统，并鉴定了4个ROS 1和2个RET新融合基因。我们还提出了一个新的实体低度恶性B细胞淋巴瘤，其特点是IRF 4重排

We identified PPFIBP1-ALK in inflammatory myofibroblastic tumor and TPM3-ALK and EML4-ALK in renal cell carcinoma. In lymphoma, we found SQSTM1-ALK. KLC1-ALK was identified from a formalin-fixed paraffin-embedded (FFPE) tissue by RACE optimized for FFPE. We established a unique system for identification of fusion genes based on histopathological methods, and identified 4 ROS1 and 2 RET novel fusion genes. We also proposed a new entity of low-grade B-cell lymphoma, which was characterized by IRF4 rearrangement