Development of Scanning Probe Electron-Enhanced Raman Scattering Microscope and its Application to Interfacial Chemistry

研制了扫描探针电子增强拉曼散射显微镜。对激光激发金属针尖产生电子的各种方法进行了实验研究。我们发现，最好的方法是将尖端引入到激光全内反射激发的近场区域。这是因为它适合于空间受限的样品室下的照明，并且易于应用于水中的各种样品。此外，FM模式与优化的光学排列相结合，使我们能够在单分子分辨率水平上进行高分辨率测量。我们已将该仪器应用于碳材料、脂类或蛋白质自组装的测量，以测试拉曼增强因子和测量机械响应的能力。

Scanning probe electron-enhanced Raman scattering microscope has been developed. Various methods for the generation of electrons from a metal tip by laser excitation were investigated experimentally. We found that the optimal method was the introduction of the tip to the near-field area excited by total internal reflection of laser beam. This is because it is appropriate for the illumination under spatially restricted sample chamber and is easy for the application to various samples in water. In addition, the combination of the FM modewith the optimized optical arrangement allowed us high-resolution measurements at single molecular resolution level. We have applied the instrument developed here to the measurements on carbon materials, lipid or protein self-assemblies for the test of the Raman enhancement factor and of the ability to measure the mechanical responses