Development of education movies demonstrating real-time biophysical phenomena by two photon laser microscopy

我曾从事用双光子激光显微镜实时展示体内和体外生物物理现象的教育电影的开发。双光子激光显微镜是一种基于双光子激发的新方法，它在动物体内产生新的光子，用于实时可视化细胞图像。这些实时电影展示了发生在我们体内的动态现象，可能会增强学习生物科学的学生的理解。在这项研究中，我获得了胃肠道、肝脏、胰腺、肾脏、大脑和血管中正常细胞的实时图像。我还观察了肠道癌细胞的实时图像，这让我有机会开发一种新方法，在超早期阶段识别非常小的癌症。在JST的资助下，新方法已作为国际专利提交。本研究获得的教育影片将在一个免费的互联网服务中打开。

I have engaged in development of education movies demonstrating real-time biophysical phenomena in vivo and in vitro by two photon laser microscopy. Two photon laser microscopy is a new method based on two photon excitation which generates new photons inside the animal body for visualizing real-time images of cells. These real-time movies demonstrating dynamic phenomena occurring inside our body probably enhance the understanding of students studying biological science. In this study I have obtained the real-time images of normal cells in gastrointestinal tracts, liver, pancreas, kidney, brain, and vessels. I have also observed the real-time images of cancer cells in intestines, which gives me an opportunity to develop a novel method to identify very small cancers at the ultra-early stages. The new method has been submitted as an international patent under the financial support from JST. The education movies obtained in this study will be opened in a free internet service.