Research on signal transduction via transcriptional mediators

我们发现辅阻遏子Ski与TRIM27结合，TRIM27通过与USP7形成复合体而促进去泛素化。研究发现，TRIM27进入细胞核是为了响应Poly(I-C)的刺激，这表明滑雪泛素化可能是由TRIM27响应特定信号而调节的。在转录接头shn-2基因敲除的小鼠中，胸腺T细胞分化被阻断。研究发现，shn-2在多个位点被O-GlcNAc修饰，从而调节shn-2的核进入。研究表明，shn-2基因定位于T细胞受体β基因，并调节等位基因排斥。

We found that corepressor Ski binds to TRIM27, and that TRIM27 contributes to deubiquitination via forming a complex with USP7. It was found that TRIM27 enters into the nucleus in response to poly(I-C) stimulation, suggesting that Ski ubiquitination may be regulated by TRIM27 in response to specific signaling.In knockout mice of transcriptional adaptor Shn-2, T cell differentiation in thymus is blocked. It was found that Shn-2 is modified by O-GlcNAc at several sites which modulates nuclear entry of Shn-2. It was demonstrated that Shn-2 is localized on the T-cell receptor beta gene locus, and modulated allelic exclusion.