Autologous Bone Marrow Stromal Cell Transplantation for Stroke: Steps to Standardize the Clinical Protocols

用人血小板裂解液(HPL)代替胎牛血清(FCS)培养人骨髓基质细胞(BMSCs)。用酶联免疫吸附试验定量检测其生长因子的产生。用超顺磁性氧化铁(SPIO)标记BMSCs。制作大鼠永久性局灶性脑缺血模型，伤后7d将骨髓间充质干细胞注入同侧纹状体内。HPL和FCS对骨髓间充质干细胞的增殖无明显影响。BMSC-HPL产生的BDNF、HGF、NGF、PDGF-BB和转化生长因子-β1的量并未减少。Rotarod实验显示缺血后运动功能下降，骨髓间充质干细胞移植促进了运动功能的恢复。MRI显示SPIO-BMSCs向病灶迁移。~(18)F-FDG PET显示葡萄糖利用率的恢复有促进作用。HPL在扩增骨髓间充质干细胞方面可能是有价值和安全的。生物成像技术的应用对骨髓间充质干细胞移植治疗中风也具有一定的应用价值。

Human bone marrow stromal cells (BMSCs) were cultured with human platelet lysate (hPL) instead of fetal calf serum (FCS). Their production of growth factors was quantified with ELISA. The BMSCs were labeled with superparamagnetic iron oxide (SPIO). Rat permanent focal ischemia models were made and BMSCs were injected into the ipsilateral striatum 7 days post-insult. There was no difference in cell proliferation between the BMSCs cultured with hPL and FCS. The BMSC-hPL produced not less amount of BDNF, HGF, NGF, PDGF-BB and TGF-beta1. Rotarod test showed that the motor function deteriorated after ischemia and BMSC transplantation enhanced the recovery. MRI demonstrated that the SPIO-BMSCs migrated towards the lesion. 18F-FDG PET showed that the recovery of glucose utilization was promoted. The hPL may be valuable and safe in expanding BMSCs. The application of bio-imaging techniques are also valuable for BMSC transplantation against stroke.