Human bone marrow stromal cells transplantation to regenerate central nervous system ; The translational research with novel technologies.

本研究旨在将人骨髓基质细胞（BMSC）的再生医学应用于中枢神经系统疾病。实验结果如下：(1)骨髓间充质干细胞可以用一些生物基质培养。(2)在培养基中添加G-CSF（粒细胞集落刺激因子）可刺激骨髓间充质干细胞的细胞活性。另一方面，人血小板裂解液（PL）的加入对动物无血清细胞培养是有益的。(3)纤维蛋白基质或Mebiol凝胶支架可作为细胞递送系统。(4)利用光学成像或MRI技术可以对移植骨髓间充质干细胞的行为进行暂时、无创的监测。

This study was aimed to translate the regenerative medicine with human bone marrow stromal cells (BMSC) to the central nervous system disorders. The results were as follows; (1) the BMSC could be cultured with some bio-matrixes. (2) The addition of G-CSF (granulocyte-colony stimulating factor) to the culture medium could stimulate the cellular activity of the BMSC. On the other hand, the addition of human platelet lysate (PL) was useful for the animal serum-free cell culture. (3) The scaffold with fibrin matrix or Mebiol gelR was useful as a cell delivery system to the lesion. (4) The behavior of the transplanted BMSC could be monitored temporally and noninvasively using the optical imaging or MRI.