Human bone marrow stromal cells transplantation to regenerate central nervous system ; The translational research with novel technologies.

本研究旨在将人骨髓基质细胞(BMSC)再生医学移植到中枢神经系统疾病中。结果表明：(1)骨髓间充质干细胞可以在一定的生物基质中培养。(2)在培养液中加入粒细胞集落刺激因子(G-CSF)可刺激BMSC的细胞活性。另一方面，添加人血小板裂解物(PL)有利于动物无血清细胞培养。(3)含纤维蛋白基质或MEBIOL GELL R的支架可作为细胞给药系统。(4)应用光学成像或MRI可对移植的骨髓间充质干细胞的行为进行时间和非侵入性监测。

This study was aimed to translate the regenerative medicine with human bone marrow stromal cells (BMSC) to the central nervous system disorders. The results were as follows; (1) the BMSC could be cultured with some bio-matrixes. (2) The addition of G-CSF (granulocyte-colony stimulating factor) to the culture medium could stimulate the cellular activity of the BMSC. On the other hand, the addition of human platelet lysate (PL) was useful for the animal serum-free cell culture. (3) The scaffold with fibrin matrix or Mebiol gelR was useful as a cell delivery system to the lesion. (4) The behavior of the transplanted BMSC could be monitored temporally and noninvasively using the optical imaging or MRI.