The cell transplant system using improved TRECK mice

TRECK方法对于建立模型小鼠和获得有用的供体细胞非常有用。然而，由于DTR是一种生长因子，其过度表达会导致小鼠出现轻微的异常表型，因此很难使DTR在小鼠全身表达。在这项研究中，为了解决这些问题，我们使用降低了生长因子活性的改良DT受体培育了改良的treck小鼠。改良的treck小鼠表现出正常的表型，DTR可以在小鼠全身表达。这一改进有望扩大treck方法的适用范围。此外，我们还可以证明，移植的和接受的供体细胞可以通过DT从受体小鼠身上再次移除，以检查移植的效果。

The TRECK method is very useful for generating model mice and obtaining useful donor cells. However, it has been difficult to make DTR expressed in whole bodies of mice because DTR is a growth factor and overexpression of it induces a slightly abnormal phenotype. In this study, to solve these problems, we generated improved TRECK mice using a modified DT receptor with reduced growth factor activity. The improved TRECK mice showed a normal phenotype and the DTR could be expressed in whole mice bodies. This improvement is expected to extend the applicable range of the TRECK method. Furthermore, we could demonstrate that transplanted and accepted donor cells from this mouse could be removed again from the recipient mouse by DT administration to examine the effect of the transplantation.