Development of biologic scaffold materials composed of extracellular matrix by means of the supercritical fluid technology with ultrasonic irradiation.

我们试图通过超临界流体技术从动物组织或器官中制备由细胞外基质(ECM)组成的生物支架材料。为了获得可以克服器官移植供体器官严重短缺的生物支架材料，在高压条件下，在液态二氧化碳中进行超声辐照，实现了导致大鼠肾、肝脏、心脏和猪等整个器官发生排斥反应和脱细胞的细胞成分的分离。用微焦点X射线CT获得脱细胞器官的结构数据。这些脱细胞器官的结构数据可能导致多种由细胞外基质组成的生物支架材料的生产，用于器官移植供体器官的工业化规模。

We attempted to generate biologic scaffold materials composed of extracellular matrix (ECM) from animal tissues or organs by means of the supercritical fluid technology. In order to obtain the biologic scaffold materials which may overcome a severe shortage of donor organs for organ transplant, the separation of cellular components leading to allograft rejection and decellularization of whole organs, such as kidneys livers, hearts of rats and pigs was achieved by ultrasonic irradiation in liquid carbon dioxide at high pressure condition. The structural data of decellularized organs were obtained by a micro-focus X-ray CT. These structural data of decellularized organs may lead to multi production of biologic scaffold materials composed of extracellular matrix for donor organs for organ transplant in an industrial scale.