Roles of a vesicular transport network and a membrane fusion in autophagy in Saccharomyces cerevisiae

我们发现，在几个SNARE(可溶性NSF附着蛋白受体)突变体中，当分泌途径被阻断时，自噬受到抑制，这表明来自分泌途径的膜脂供应可能对自噬的形成起重要作用。我们还在自噬前结构中发现了一些与ATG9直接相关的SNARE蛋白，提示这些蛋白可能是自噬小体形成的机制。

We found that autophagy was inhibited when the secretory pathway was blocked in several SNARE (soluble NSF attachment protein receptor) mutants, suggesting that a supply of membrane lipid from the secretory pathway might be important for autophagosome formation. We also showed that some SNARE proteins directly associated with Atg9, which plays roles in autophagosome formation, at preautophagosomal structure, suggesting that these proteins might function as machinery of autophagosome formation.