Basic research on persistent infection of HCV and molecular mechanisms of RNA interference

TRAX （Translin-associated factor X）被认为是RISC （RNA诱导沉默复合体）的一个组成部分，参与RNA干扰（RNAi）。鉴于TRAX在RNAi机制中的重要作用，我们分析了其对丙型肝炎病毒（HCV）持续感染机制的影响。虽然我们在研究期间无法证明TRAX参与HCV感染，但我们对TRAX分子的性质得出以下结论：1)TRAX蛋白不能单独在细胞中表达。2)TRAX蛋白通常以与Translin蛋白复合物的形式存在，以逃避泛素非依赖性蛋白酶体降解途径3)TRAX蛋白的c端区域易被降解。

TRAX (Translin-associated factor X) is known as a component of RISC (RNA-induced silencing complex) that is involved in RNA interference (RNAi). Given the significant role of TRAX on RNAi machinery, we analyzed its effect on the mechanism of persistent infection of hepatitis C virus (HCV). Although we could not prove the involvement of TRAX in HCV infection during the study period, we obtained the following results for the nature of TRAX molecule: 1)TRAX protein alone cannot be cellularly expressed. 2)TRAX protein usually exists as a complex with Translin protein to escape from ubiquitin-independent proteasome degradation pathway 3) The C-terminal region of TRAX protein is susceptible to degradation.