A basic study for evaluation method of brain function on forensic autopsy cases.

采用免疫组化染色法检测痴呆脑组织中与神经元变性、细胞损伤和氧化应激相关的17种抗体。特别是，大量spd被YKL-39、Tau、CS和HSP70抗体染色。NOS与GFAP、NOS与CS、ApoE与CS、Tau与HSP70、CS与HSP70的平均spd发生频率呈正相关。用激光共聚焦显微镜多荧光免疫组织化学染色检测同一SPD中与痴呆相关的物质。虽然SPD与精神分裂症的神经元凋亡有关，但SPD可能不仅来自神经元变性，还可能来自退行性痴呆的神经胶质。早期检测到EAAC1和/或α -syn反应性与神经元变性相关的SPDs，中晚期检测到S-100和/或YKL-40反应性与星形胶质细胞相关的SPDs。

Autopsied brains with dementia were examined by means of immunohistochemical staining method using 17 antibodies, related with neuronal degeneration, cellular damage and oxidative stress. Especially, a large number of SPDs were stained by antibodies of YKL-39, Tau, CS and HSP70. Furthermore, development frequency of SPDs between by mean of NOS and GFAP, NOS and CS, ApoE and CS, Tau and HSP70, and CS and HSP70, showed positive correlations. Substances relating with dementia are detected in same SPD by mean of multi fluorescent immunehistochemical staining visualized by confocal laser microscopy. Although, SPD were related with apoptosis of neurons in schizophrenia, SPD might come from not only neuronal degeneration but also glial in degenerative dementia. SPDs with reactivity of EAAC1 and/or alpha-syn related with neuronal degeneration were detected in the early stages and they with reactivity of S-100 and/or YKL-40 related with astrocytes were detected in the middle or later stages.