A role of NETs for damage of renal glomerular endothelial cells in ANCA-associated renal vasculitis

ANCA相关性血管炎的肾活检病理学检查表明，肾小球内有MPO阳性细胞浸润沿着细胞外沉积。称为NET的染色质纤维从中性粒细胞释放。已知NET与ANCA相关性血管炎（AAV）的发病机制有关。我们观察了NETs的形成，以探讨NETs在AAV中的作用。AAV患者的外周血中性粒细胞用佛波豆蔻酸酯醋酸酯（PMA）致敏，并计算产生NETs的细胞的百分比。活动性AAV患者的中性粒细胞比对照组的中性粒细胞产生更多的纤维样NET。AAV处理后，纤维样NETs的释放明显减少。这些数据表明，中性粒细胞在血管内皮上释放NET对于AAV的发病机制是重要的。

Pathological examination of renal biopsies from ANCA-associated vasculitis indicated that there was a glomerular infiltration of MPO-positive cells along with deposition of extracellular. Chromatin fibers called NETs are released from neutrophils. It has been known that NETs are implicated in the pathogenesis of ANCA-associated vasculitis (AAV). We observed NETs formation in order to investigate the role of NETs in AAV.Peripheral blood neutrophils from AAV patients were primed with phorbolmyristate acetate(PMA) and the percentage of NETs producing cells were calculated. Neutrophils from active AAV patients produced much more fiber-like NETs than neutrophils from controls. The release of fiber-like NETs was markedly decreased by treatment for AAV. These data suggest the release of NETs by neutrophil at vascular endothelium is important for the pathogenesis of AAV.