Development of the integrated method for the analysis of brain function by high resolution PET for small animals with the aid of high field MRI

小动物高分辨率PET有望成为阐明疾病病理生理和创新药物开发的重要工具之一。在这项研究中，我们试图开发一个系统，使我们能够分析分子目标成像的微pet利用高场MRI。我们采用一种可用于小动物微pet和高场MRI的新型小鼠头部固定装置，对C57BL小鼠进行MRI和FDG-PET检查，并将每只小鼠的MRI归一化到标准小鼠脑图谱后，对微pet成像进行解剖描绘。我们能够将FDG- pet的功能图像叠加在解剖图谱上，并计算FDG的区域摄取超过全球平均脑摄取。我们发现自动化方法和手工方法之间有很好的相关性。结果表明，自动区域分析可应用于正常和转基因小鼠脑FDG-PET成像。

The high-resolution PET for small animals is expected to be one of the important tools for the elucidation of the pathophysiology and the innovative drug development of the disease. In this study, we tried to develop the system which enables us to analyze molecular target imaging by micro-PET using high-field MRI. Using a new head fixation device for mouse which can be used commonly both for micro-PET and high-field MRI for small animals, we underwent both MRI and FDG-PET of C57BL mice and made an anatomical delineation on micro-PET imaging after normalization of MRI of each mouse to standard mouse brain atlas. We were able to superimpose a functional image of FDG-PET on an anatomical atlas and calculate regional uptake of FDG over global mean brain uptake. We have found that there was a good correlation between automated method and manual method. It is concluded that automated regional analysis can be applied to the FDG-PET imaging of normal and transgenic mouse brain.