Development of a new fluorescence imaging system for peritoneal and lymph node metastasis of GI tract cancers

腹膜和淋巴结转移是胃癌根治术后最常见的复发方式。然而，这种复发很难通过常规的CT和MRI在早期发现。在本研究中，我们首先利用GFP标记的肿瘤细胞建立了一种新的淋巴转移模型。其次，我们利用ICG标记的抗体作为探针，开发了一种新的用于检测转移的腹腔镜荧光装置。利用这些小鼠转移模型和成像设备，我们成功地对腹膜转移和淋巴结转移进行了早期检测，具有较高的敏感性和特异性。

Peritoneal and lymph node metastasis is the most frequent pattern of recurrence after curative surgery for gastric cancer. However, such a recurrence is difficult to detect by conventional CT and MRI at an early stage. In the present study, we first developed a new lymph node metastasis model using GFP-tagged tumor cells. Second, we developed a new laparoscopic fluorescence device for detecting metastasis using ICG-labeled antibody as a probe. Using these mouse metastasis model and imaging device, we successfully detected peritoneal metastasis and lymph node metastasis at an early stage with high sensitivity and specificity.