Pre-clinical study on pulmonary perfusion preservation using modified ET-Kyoto solutions

为探讨改良ET-K液作为肺保存液的临床应用价值，我们采用大鼠和犬的体外肺灌流模型，评价了持续灌流对肺保存的影响。无论是去钾的ET-K组还是不含HES的ET-K组，对于可靠的临床实践都没有足够的肺保护作用。此外，在灌注液中添加红细胞也没有显示出任何明显的保存效果改善。临床应用还需要进一步的大范围改进。

We evaluated the effects of continuous perfusion on pulmonary preservation using a rat and a dog ex vivo perfusion models aiming at clinical application of modified ET-K solution as a perfusate. Neither a potassium-reduced ET-K nor an ET-K without HES demonstrated sufficient pulmonary preservation for reliable clinical practice. In addition, supplementation with red blood cells to the perfusion solution also failed to show any significant improvements of the preservation effect. A further extensive modification is needed for the clinical application.