Role of mTOR signaling pathway for the growth and proliferation of uterine leiomyoma

我们研究了哺乳动物靶向雷帕霉素(MTOR)信号通路在子宫肌瘤生长和增殖中的作用，但没有发现与子宫肌瘤组织相比mTOR信号通路相关基因的蛋白水平和mRNA水平的过表达。非肥胖糖尿病/严重联合免疫缺陷小鼠异种移植模型显示，异基因移植物在肾被膜下呈性别激素依赖性生长，雌激素和孕激素受体均为阳性表达，与原始肌瘤组织相比具有相似的代表性基因表达。在该模型中，我们只使用非遗传性人工毛发原代培养的子宫肌瘤细胞；因此，我们认为该模型是研究和开发子宫肌瘤新疗法的良好工具。

We investigated the role of mammalian target of rapamycin (mTOR) signaling pathway for the growth and proliferation of uterine leimyoma; however, we did not find any overexpression of protein levels as well as mRNA levels of mTOR signaling pathway-related genes in uterine leiomyoma compared with those in myometrial tissues.Next, we established a novel in vivo xenograft model for uterine leiomyoma, and evaluated its characters. The non-obese diabetic/severe combined immunodeficient murine xenograft model has shown a sex-steroid dependent growth of xenograft beneath the kidney capsule, a positive expression for both estrogen and progesterone receptors, and a similar representative gene expression compared with original leiomyoma tissue. In this model, we use only non-genetic manupilated primary cultured leiomyoma cells; therefore, we believe this model is a good tool for the research and the development of new therapies for uterine leiomyoma.