Analysis of virulence regulatory mechanism of group A streptococcal ncRNA

化脓性链球菌调节感染部位毒力因子的表达，并引起化脓性疾病。最近，RNA的转录和翻译调控已被注意到。在这项研究中，非编码RNA的候选人在化脓性链球菌中寻找，并使用构建的突变株研究非编码RNA的靶mRNA。此外，关于编码转录调节因子的必需基因，在该基因的上游引入核糖开关，并分析必需基因的功能。当突变株中该基因的翻译速率降低时，生长受到明显抑制。此外，突变体表现出异常的细胞形态和不规则的表面结构。我们的研究结果表明，核糖开关为基础的翻译控制是有用的化脓性链球菌的基本因素的分析。

Streptococcus pyogenes regulates expression of virulence factors in infection sites, and causes purulent diseases. Recently, transcriptional and translational regulations by RNA have been noted. In this study, non-coding RNA candidates were searched in Streptococcus pyogenes, and target mRNAs of non-coding RNAs were investigated using constructed mutant strains. Moreover, regarding the essential gene encoding a transcriptional regulator, an riboswitch was introduced upstream of the gene, and the function of the essential gene was analysed. When the translation rate of the gene was reduced in the mutant strain, the growth was markedly inhibited. Moreover, the mutant showed an abnormal cellular morphology and irregular superficial structure. Our results indicate that riboswitch-based translational control is useful for analysis of essential factors of Streptococcus pyogens.