DEVELOPMENT OF IMMUNOASSAY FOR PESTICIDE RESIDUES IN IMPORTED AGRICULTURAL PRODUCTS USING MONOCLONAL ANTIBODY

传统的气相色谱、高效液相色谱和质谱等仪器分析方法准确度高，但需要大量的仪器设备和熟练的技术人员。利用单克隆抗体，建立了一种简便、灵敏度高的酶免疫分析方法，用于环境样品和进口农产品中农药残留的分析。农药的生产和这些农药的测定性能进行了优化。农药涕灭威和灭多威的工作范围为10- 1000 ng/ml，涕灭威磺隆和甲基敌百虫的工作范围为1-100 ng/ml，氯苯胺灵的工作范围为0.5-10 ng/ml。（水和土壤）和农产品（马铃薯、橙子、卷心菜）中农药的回收率较高，不需要净化处理，该方法可用于水样和农产品中农药残留的检测，或者可以与仪器方法结合使用。

The conventional pesticide residue analyzes by instrumental methods using gas chromatography, high performance liquid chromatography and mass spectrometry are accurate, but require time consuming, expensive equipments and skilled technicians.In this rsearch, we developed the rapid, simple and high sensitive enzyme immunoassay for pesticide residue analyzes in environmental samples and imported agricultural products using monoclonal antibodies.Monoclonal antibodies against several pesticides were produced and the assay performances for these agrochemicals were optimized. The working ranges of assay were 10-1,000 ng/ml for insecticide aldicarb and methomil, 1-100 ng/ml for aldicarb sulfon and parathion methyl, and 0.5-10 ng/ml for herbicide chlorpropham.The results of measurement of pesticides in environmental samples (water and soil) and agricultural products (potato, orange, cabbage) spiked with pesticides showed high recovery without cleanup procedure.This immunoassay for pesticide residue will be applicable to monitoring of residue in water samples and agricultural products, or can be used in conjunction with instrumental methods.