Dual-modality imaging with Tc-99m and fluorescent indocyanine green using surface-modified silica nanoparticles for the image of malignant tumors

我们的目的是开发双模态成像探针与Tc-99 m和荧光染料使用表面改性的二氧化硅纳米粒子的恶性肿瘤的图像。用吲哚菁绿色与聚酰胺胺包覆的二氧化硅纳米颗粒（PCSN）偶联，然后用Tc-99 m标记。将抗HER 2抗体与标记的PCSN共价结合，将双模态成像探针经尾静脉注射到裸鼠移植瘤体内。异种移植的SKBR 3肿瘤，HER 2过表达肿瘤，显示高荧光信号强度，与其中HER 2表达低的异种移植的MDA-MB 231肿瘤中的低信号强度形成对比。与SKBR 3肿瘤相比，SKBR 3肿瘤中的放射性也增加。结果表明，这种双模态成像探头可能是有益的描绘高荧光病变与解剖结构的外科手术后，探测深部病变的γ射线。

We aimed to develop dual-modality imaging probes with Tc-99m and fluorescent dyes using surface-modified silica nanoparticles for the image of malignant tumors. Polyamidoamine coated silica nanoparticles, PCSNs, were conjugated with indocyanine green and then labeled with Tc-99m. Anti-HER2 antibodies were covalently combined with the labeled PCSNs.The dual-modality imaging probes were injected in athymic mice with xenografted breast tumors through the tail vain. The xenografted SKBR3 tumors, HER2-overexpressing tumors, showed high fluorescence signal-intensities contrast to the low signal-intensities in the xenografted MDA-MB231 tumors in which HER2 expression was low. Radioactivity in the SKBR3 tumors also increased compared to that in the SKBR3 tumors. The results revealed that this dual-modality imaging probes may be beneficial to delineate the high-fluorescent lesions with anatomical configurations in surgical procedures after detection of the deep situated lesions by gamma rays.