Optimization of bone regeneration by use of mesenchymal stem cells and its application for closure of jaw cleft

我们的研究目的是利用骨髓间充质干细胞（MSCs）和一种新的支架，碳酸羟基磷灰石（CAP）建立颌骨裂骨再生。在比格犬的上颌骨两侧制造人工颌裂。用抽吸针从髂骨骨髓中提取间充质干细胞，并进行培养。1个月后，将MSCs和新型支架材料CAP颗粒粉移植到人工骨缺损中。MSCs/CAP移植3个月后颌骨裂区骨再生成功。研究还表明，破骨细胞能够快速消化CAP颗粒。此外，还实现了正畸牙齿向再生骨的移动。强调MSCs/CAP移植治疗作为CLP颌骨裂骨缺损患者骨再生的一种新的治疗方式具有很大的潜力。

The purpose of our study is to establish bone regeneration of jaw cleft by use of bone marrow-derived mesenchymal stem cells (MSCs) with a new scaffold, carbonated-hydroxyapatite (CAP). Artificial jaw cleft was created on the both side of maxilla in Beagle dogs. MSCs were derived from bone marrow of iliac bone by use of an aspiration needle, and cultured. After 1 month, MSCs and new scaffold material, CAP granulated powder, were transplanted into the artificial bone defects. Bone regeneration at jaw cleft site was achieved successfully by the transplantation of MSCs/CAP after 3 months. It is also demonstrated suggested that CAP particles could be digested rapidly by osteoclasts. In addition, orthodontic tooth movement into regenerated bone was also achieved. It is emphasized that MSCs/CAP transplantation therapy has a great potential as a new treatment modality for bone regeneration in CLP patients with bone defect due to jaw cleft.