Development of high-performance microchip for DNA amplification towards sigle-cell genome analysis

我们成功地将耐热DNA聚合酶固定在7种不同孔径的介孔二氧化硅上，并保持了足以扩增目的DNA的酶活性。介孔二氧化硅载体能够简单、选择性和优先地固定化DNA聚合酶，并通过优化孔径来调节从少量底物DNA中进行DNA扩增的酶活性。此外，含有酶和介孔二氧化硅复合材料的微流控芯片具有精确、高效和连续扩增目的DNA的特点。因此，这些数据显示了介孔二氧化硅作为酶反应平台的巨大潜力，不仅可以用于常规的DNA扩增，还可以从少量样品中进行高效的DNA扩增，例如几个DNA分子。

We successfully immobilized thermostable DNA polymerase on seven types of mesoporous silicas with different pore diameters and retained enzymatic activity sufficient for amplifying target DNA. The mesoporous silica support enabled the simple, selective, and preferential immobilization of DNA polymerase and the regulation of enzymatic activity for DNA amplification from small amounts of substrate DNA by optimizing pore size. In addition, the microfluidic chip containing composites of the enzyme and mesoporous silica exhibited precise, efficient, and continuous amplification of target DNA. Thus, these data show the great potential of mesoporous silica as enzymatic reaction platforms not only for conventional DNA amplification but also for efficient DNA amplification from small amounts of sample such as several molecules of DNA.