Live-imaging probe for strain-selective labeling of bacteria

我们开发了一种利用化学合成的细胞壁（肽聚糖）前体衍生物在细菌表面显示各种分子的方法。在这项研究中，我们重点研究了菌株对细菌表面的特异性修饰。大肠杆菌O157在其o抗原多糖中含有罕见的perosamine部分。我们选择了一种钙胺前体，作为对大肠杆菌O157进行选择性表面修饰的基础。包括大肠杆菌O157在内的6株菌株在含有类似物的培养基中孵育，并用荧光团标记细菌表面的叠氮基团。流式细胞术分析显示大肠杆菌O157的选择性标记。

We have developed an approach to display various molecules on the bacterial surface using a chemically synthesized cell-wall (peptidoglycan) precursor derivative. In this research, we focused on strain-specific modification of the bacterial surface. E. coli O157 has an uncommon perosamine moiety in their O-antigen polysaccharide. We chose a precursor of perosamine, as a base for selective surface modification of E. coli O157. Six bacterial strains including E. coli O157 were incubated in growth media containing the analog, and the azido groups on the bacterial surface were labeled with a fluorophore. Flow cytometry analysis showed the selective labeling of E. coli O157.