Basic studies of improved cryopreservation using supercooling technology.

对法国卤虫(A.franciscana)的包囊进行了水化处理，并测定了其孵化率，以评估不同冷冻速度对细胞和组织可能造成的损伤。我们观察到，快速冷冻(浸泡在液氮中)的水合囊的孵化率在短期内下降，但在缓慢的冷冻速度(0.5C/min)下保持了较高的孵化率，并通过海藻糖和二甲基亚砜(DMSO)等冷冻保护剂的处理提高了脱壳的水合囊的孵化率。我们还利用扫描电子显微镜和冷冻转移系统(Cryo-SEM)对冷冻水化囊泡的表面进行了评价，发现两种冷冻速度下的胚胎切片上存在着细微的结构差异。

The cysts of Artemia franciscana (A. franciscana) were hydrated and their hatching rates were measured in order to estimate possible damagaes of cells and tissues by various freezing speeds. We observed that the hatching rates of hydrated cysts declined shortly at rapid frozen (submerged in liquid nitrogen), but high hatching rates maintained at slow freezing rate (0.5C/min), and that improved hatching ratio of decapsulated hydrated cysts by the treatment of cryoprotectants such as trehalose and dimethl sulfoxide (DMSO). We also evaluated that the surface of frozen hydrated cysts using a scanning electron microscope attached with cryo-transfer system (Cryo-SEM), and found fine structural discrepancy in the section of embryos between two freezing speeds.