Molecular probes to visualize cellular processes in vivo

在这项研究中，一个合理的发展超发光变种的荧光素酶来自高斯princeps的证明。一个推定的催化中心的高斯荧光素酶的指定和修饰的定点突变。发现有效的变体产生高达10倍强的生物发光，与天然GLuc相比，天然腔肠素发射高达33 nm的红移，在生物测定中呈现有效的光学特征。用BALB/c裸鼠中的鼠B16黑素瘤转移证明了有利的性质。

In this study, a rational development of superluminescent variants of a luciferase derived from Gaussia princeps was demonstrated. A putative catalytic center of Gaussia luciferase was assigned and modified by a site-directed mutagenesis. The potent variants were found to generate up to 10 times stronger bioluminescence, emitting red shifts of up to 33 nm with natural coelenterazine than native GLuc, rendering an efficient optical signature in bioassays. The advantageous properties were demonstrated with metastases of murine B16 melanoma in BALB/c nude mice.