Molecular probes to visualize cellular processes in vivo

在这项研究中，一个合理的发展的超发光变体的荧光素酶源自高斯红被证明。一个假定的高斯荧光素酶催化中心被指定并通过位点定向诱变修饰。研究发现，与天然GLuc相比，天然coelenterazine的有效变体产生的生物发光强度高达10倍，发出高达33 nm的红移，在生物分析中提供了有效的光学特征。在BALB/c裸鼠B16黑色素瘤的转移中证实了这些有利的特性。

In this study, a rational development of superluminescent variants of a luciferase derived from Gaussia princeps was demonstrated. A putative catalytic center of Gaussia luciferase was assigned and modified by a site-directed mutagenesis. The potent variants were found to generate up to 10 times stronger bioluminescence, emitting red shifts of up to 33 nm with natural coelenterazine than native GLuc, rendering an efficient optical signature in bioassays. The advantageous properties were demonstrated with metastases of murine B16 melanoma in BALB/c nude mice.