The mechanisms of induction of autophagic cell death by histone deacetylase inhibitor SAHA

在电子显微镜下，SAHA诱导多药耐药骨肉瘤细胞自噬。SAHA还以剂量依赖方式诱导自噬相关蛋白Beclin1和Lc3-I、II。这些事实表明，SAHA诱导多药耐药骨肉瘤细胞自噬细胞死亡。接下来，我们研究了SAHA治疗后骨肉瘤亲本细胞和多药耐药细胞的细胞周期分布。SAHA使亲本细胞中的Sub-G1期细胞比例增加，而多药耐药细胞中的G2/M期细胞比例增加。因此，我们研究了自噬与G2/M期的关系。因此，我们得到了SAHA诱导自噬和G2/M期同时存在的新事实

In electron microscope SAHA induced autophagosome in multidrug-resistant osteosarcoma cell. SAHA also induced autophagy related protein Beclin1 and LC3-I 、II in dose dependent manner. These facts demonstrated that SAHA induce autophagic cell death in multidrug-resistant osteosarcoma cell. Next, we investigated cell cycle population in osteosarcoma parental cell and multidrug-resistant cell after SAHA treatment. SAHA increased Sub-G1 fraction in parental cell, but G2/M fraction in multidrug-resistant cell. Therefore, we investigated the relationship between autopphagy and G2/M phase. As a result, we got new fact that SAHA induce autophagy and G2/M phase at the same time