Roles of soluble VEGF receptor on angiogenesis of eye in vivo

在这里，我们展示了调节可溶性Flt-1替代3'端加工的机制，Flt-1是一种潜在的抗血管生成因子。我们发现hnRNP D通过结合Flt-1前mrna的内含子13上的调控序列下调可溶性Flt-1的表达。此外，我们还进行了慢病毒介导的精原干细胞体内转基因。由于慢病毒颗粒不能感染精原干细胞，提示该方法效果不佳。因此，我们将常规地构建转基因小鼠，并在体内展示可溶性Flt-1在眼血管生成中的作用。

Here, we showed the mechanisms for regulating alternative 3'-end processing of soluble Flt-1, a potential antiangiogenic factor. We found that hnRNP D down-regulated soluble Flt-1 expression by binding to the regulatory sequence on intron 13 of Flt-1 pre-mRNA. Furthermore, we carried out the lentiviral mediated transgenesis by in vivo manipulation of spermatogonical stem cell. It was suggested that the method was not effective because lentiviral particle couldn't infect to spermatogonical stem cell. Therefore, we are going to create a transgenic mouse conventionally and show the roles of soluble Flt-1 on angiogenesis of eye in vivo.