Analysis of autophagosome formation using CLEM tomography

当细胞面临饥饿、异常蛋白积累、细菌入侵等情况时，细胞的一种生存机制即自噬被激活。自噬体（AP）在细胞质内新形成，与溶酶体融合降解。AP在细胞内形成的位置一直是一个长期存在的问题，因为这将导致其膜源。我们确定该位点为er -线粒体接触位点（Nature, 2013）。AP地层是动态的，这一发现将有助于描述地层中最有趣的部分。

One of the cell survival mechanism called autophagy gets activated when cells face starved condition, accumulates abnormal proteins, bacterial invasion and so on. Autophagosome (AP) is formed newly within cytosol that gets fused with lysosomes for degradation. Where AP is formed within cells have been a long-standing question since that will leads to its membrane source. We identified such site to be ER-mitochondria contact site (Nature, 2013). AP formation is dynamic and this finding will help characterizing the most interesting part of formation.