Screening of anti-virus activities from deep-sea-adapted bacteria

我们从日本海、相模湾和日本海沟采集的深海泥浆样本中分离出适应深海的细菌。将这些细菌作为细菌文库保存，并根据16S rDNA核苷酸序列分析其多样性。培养1000个细菌，保存上清液，用于抗病毒活性筛选。此外，成功建立了用于抗病毒活性筛选的EGFP/NLS/NES细胞系。此外，我们发现13种深海细菌培养物的上清混合物具有显著的抗流感病毒活性。

We isolated deep-sea-adapted bacteria from deep-sea-mud samples collected at the Japan sea, Sagami bay, and Japan trench. These bacteria were stored as a bacterial library and their diversity was analyzed based on the 16S rDNA nucleotide sequences. One thousand bacteria were cultured and the resulting supernatants were stored so as to be used for anti-virus activity screening. In addition, establishment of EGFP/NLS/NES cell line which will be used for anti-virus activity screening was successfully achieved. Furthermore, we found significant anti-influenza virus activities in13 supernatant mixtures of deep-sea bacterial cultures.