Prevention studies for diabetic complications based on antioxidative proteins specifically expressed in cats with Diabetes.

糖尿病（DM）是一种公认的内分泌/代谢疾病。众所周知，猫的糖尿病并发症，包括白内障、血管病变和神经病变，与人类和犬的糖尿病并发症相比非常轻微。为了阐明猫出现轻度糖尿病并发症的原因，检查了内源性抗氧化活性。糖尿病猫（n=17）的血浆具有较高的羟自由基清除活性比年龄和体重匹配的健康对照组（n=16）。糖尿病猫（n=3）和对照猫（n=3）的血浆经透析柱分离后，在分子量> 10 kDa的组分中发现有清除羟自由基的活性。差示血浆蛋白电泳和质谱（TOF-MS）显示触珠蛋白作为羟基自由基清除剂的候选者。血浆结合珠蛋白浓度与羟自由基清除活性呈正相关。因此，结合珠蛋白可能作为一种抗氧化剂在糖尿病猫体内发挥作用，对糖尿病并发症有一定的预防作用。

Diabetes mellitus (DM) is an well described endocrine/metabolic disease. In cats, it is also well known that diabetic complications including cataract, vasculopathy and neuropathy are very mild compared to those in humans and dogs. To elucidate the reason for the mild diabetic complications in cats, endogenous antioxidative activities were examined. Blood plasma from DM cats (n=17) possessed higher hydroxyl radical-scavenging activities than those from age- and body weight-matched healthy controls (n=16). When the blood plasma from DM cats (n=3) and controls (n=3) were fractionated with dialysis columns, the hydroxyl radical-scavenging activity was found in the MW > 10kDa fraction. Differential plasma protein electrophoresis and mass spectrometry (TOF-MS) revealed haptoglobin as a candidate of hydroxyl radical-scavenger. Moreover, plasma haptoglobin concentrations and hydroxyl radical-scavenging activities showed positive correlation. Hence, haptoglobin may work as an antioxidant in DM cats, which may preventive to diabetic complications in part.