Development of biofabrication techniques for living cells towards cell and tissue engineering

为了最大限度地利用蛋白质和活细胞等生物资源的功能，发展生理条件下的制备工艺具有重要意义。在这个项目中，能够承受活细胞脆弱性的生物制造技术向体外细胞和组织工程方向发展。微流体技术、软光刻技术和表面工程与水凝胶和聚合物纳米片的结合，使能够控制细胞环境的2D和3D细胞培养系统的发展成为可能，并且证明这些系统对于包括肌肉在内的功能组织的形成是有用的。

Developing fabrication process under physiological conditions is of significance in order to utilize the maximum function of biosources such as proteins and living cells. In this project, biofabrication techniques to endure the fragility of living cells were developed towards in vitro cell and tissue engineering. The combination of microfluidic technique, soft lithographic technique, and surface engineering with hydrogels and polymeric nanosheets enabled the development of 2D and 3D cell culture systems capable of controlling cellular environments and it is demonstrated that these systems are useful for the formation of functional tissues including muscles.