In situ measurements of the electrostatic properties inside photosynthetic reaction centres: correlation with the energy conversion function of the protein

光化学反应中心是参与能量转换的关键蛋白质复合体。它通过跨膜电荷分离将太阳能转化为化学能。电子和质子转移的偶联是在泛醌辅助因子水平上催化的。为了了解这种辅因子的氧化还原特性是如何受到蛋白质环境的微调的，我们计划使用一种电压敏感的荧光染料来探测泛醌位置。这一令人兴奋的多学科项目将有助于理解蛋白质基质如何影响和控制它们的辅因子的中点氧化还原潜力以及它们的环境。

The photochemical reaction centre is a key protein complex involved in energy conversion. It converts solar energy into chemical energy as a transmembrane charge separation. Coupling of electron and proton transfer is catalysed at the level of a ubiquinone cofactor. In order to understand how the redox properties of this cofactor are fine tuned by the protein environment, we plan to probe the ubiquinone site using a voltage-sensitive fluorescent dye. This exciting multidisciplinary project will contribute to the understanding of how protein matrices influence and govern the midpoint redox potential of their cofactors and the environments of their