Preparation of novel durable gene expression system comprising biodegradable device containing DNA complex particles coated by acidic polysaccharides

在本研究中，我们使用可注射的自形成海藻酸盐凝胶制备了持久的基因表达系统，该凝胶包括透明质酸包被的DNA复合物。当将包含用透明质酸包被的微细分散的DNA复合物的藻酸盐溶液注射到活体中时，在注射部位形成凝胶。凝胶在肿瘤组织中持续降解。通过加入无定形磷酸钙（ACP）可以控制凝胶降解和DNA复合物释放的速率。当将含有DNA复合物的藻酸盐溶液与精氨酸基因和ACP注射到小鼠肿瘤组织附近时，该制剂显示出高的治疗效果。它有望作为可注射的持续基因表达装置应用。

In this study, we prepared the durable gene expression systems using injectable auto-forming alginate gel including DNA complexes coated by hyaluronic acid. When the alginate solution including the finely dispersed DNA complexes coated with hyaluronic acid was injected in a living body, a gel was formed in the injected site. The gel continuously degraded in a tumor tissue. The rate of gel-degradation and release of the DNA complexes could be controlled by addition of amorphous calcium phosphate (ACP). When the alginate solution containing the DNA complexes with cytokine-gene and ACP was injected close to the tumor tissue of the mice, the formulation showed high therapeutic effect. It is expected for applications as an injectable sustained-gene expression device.