Engineering enzymes controlling plant polysaccharide properties

该项目旨在使用数据来确定核苷酸糖的合成和相互转化是如何调节的，以及这如何控制经济上重要的植物中阿拉伯木聚糖的性质。阿拉伯木聚糖的饮食消费减少慢性疾病。此外，阿拉伯木聚糖的属性影响加工植物生物质的成本。然而，植物多糖阿拉伯木聚糖的性质的遗传控制尚未得到解决。在将来自光合作用的碳分配成阿拉伯木聚糖中的主要控制点是糖核苷酸互变酶的活性。为了研究这些酶，将结合基因组学、糖组学和酶动力学数据，并在转基因植物中修饰靶酶。

This project will aim to use data to define how the synthesis and interconversion of nucleotide sugars is regulated and how this controls the properties of arabinoxylan in economically important plants. Dietary consumption of arabinoxylan reduces chronic diseases. Additionally, the attributes of arabinoxylan influence the cost of processing plant biomass. However, genetic control of the properties of the plant polysaccharide arabinoxylan is unresolved. A major control point in the partitioning of carbon from photosynthesis into arabinoxylan is the activity of sugar nucleotide interconverting enzymes. To characterise these enzymes, genomic, glycomic and enzyme kinetic data will be combined and the target enzymes will be modified in transgenic plants.