Response of intestinal macrophage to intestinal bacteria

本研究旨在阐明肠道巨噬细胞识别和消除外来物质的机制，并鉴定非致病性大肠杆菌、副溶血性弧菌、金黄色葡萄球菌和干酪乳杆菌诱导的选择性肠道巨噬细胞的吞噬、杀菌活性和细胞因子产生等反应。大肠杆菌、副溶血性弧菌、金黄色葡萄球菌对肠巨噬细胞的吞噬作用明显低于大肠杆菌、副溶血性弧菌。肠道巨噬细胞对不同细菌种类的杀菌活性存在差异。大肠杆菌、副溶血性弧菌和金黄色葡萄球菌刺激诱导的tnf - α和IL-12产量显著高于LPS诱导的，而非干酪乳杆菌。LPS和所有细菌的IL-10产量相同。肠巨噬细胞中tnf - α和IL-12的产生可能与吞噬作用有关。这些结果证实了肠道巨噬细胞对不同的细菌种类有不同的反应。

The study aimed to clarify the mechanism of foreign substances recognition and elimination by intestinal macrophages and to identify selective intestinal macrophage responses, such as phagocytosis, bactericidal activity, and cytokine production, induced by nonpathogenic E. coli, V. parahaemolyticus, S. aureus, and L. casei. The phagocytosis of L. casei by intestinal macrophages was significantly lower than that of E. coli, V. parahaemolyticus, S. aureus. The bactericidal activities of intestinal macrophages differed for different bacterial species. TNF-alpha and IL-12 production induced by E. coli, V. parahaemolyticus, and S. aureus stimulation was significantly higher than that induced by LPS but not L. casei. IL-10 production was same for LPS and all bacteria. The production of TNF-alpha and IL-12 in intestinal macrophages may be related to phagocytosis. These results confirmed that intestinal macrophages show different responses to individual bacteria specie.