Dynamics of interphase chromosome territories and occurrence of homologous pairing of Arabidopsis chromosomes

利用具有染色体特异性的BAC组分的FISH技术成功地对拟南芥的全部染色体和近缘物种的同源染色体进行了差异绘制。基于这一前提，在不同细胞周期和不同发育阶段的两个不同染色体数目的拟南芥物种(A.thaliana，x=5和A.arenosa，x-8)的分离核上绘制间期染色体区域(CTs)和带有染色体特异序列的FISH，将首次揭示CTs在整倍体植物中的空间排列和动态。特别是，通过将实验结果与随机CT排列和准时同源配对的模型模拟相比较，将阐明体细胞同源配对的发生和潜在的动力学。突变体、诱变剂处理和转基因品系将被用于测试修饰的重组行为和DNA损伤与随机模拟预测的间期CT的结构和排列以及同源基因相互易位频率的潜在相关性。

Differential painting of entire Arabidopsis thaliana chromosomes and of homeologous chromosomes of related species by FISH with chromosome-specific BAC contiges has been established successfully. Based on this prerequisite, painting of interphase chromosome territories (CTs) and FISH with chromosome-specific sequences on isolated nuclei of different cell cycle and developmental stages of two Arabidopsis species with different chromosome numbers (A. thaliana, x=5 and A. arenosa, x-8) should reveal for the first time the spatial arrangement and dynamics of CTs in euploid plants. In particular, the occurrence and potential dynamics of somatic homologous pairing will be clarified by comparison of the experimental results with model simulations of random CT arrangements and punctual homologous pairing. Mutants, mutagen treatments and transgenic lines will be applied to test for potential correlations of modified recombination behaviour and of DNA damage with the structure and arrangement of interphase CTs as predicted from random simulations and by the frequency of homologopus reciprocal translocations.