Demelopment of a realtime PCR-based semen identification method using the semen-specific hypomethylated DNA region

在这项研究中，我们用亚硫酸氢盐序列分析了不同体液的老化斑点，以了解先前报道的精液特异性低甲基化区域DACT1的甲基化状态。结果表明，每个旧斑点的甲基化状态都是稳定的，这可以作为法医精液鉴定的有用标志。我们进一步建立了TaqMan实时定量PCR检测方法，使DACT1区域甲基化状态的分析变得更加容易。该方法可作为精液鉴定的一种有用的辅助工具，特别是与陈旧性斑块的鉴别。

In this study, we analyzed aged stains of various body fluids by bisulfite sequence analysis for the methylation status in the previously reported semen-specific hypomethylated region, DACT1. The results indiate that the methylation status is stable in each old stain and that this can be a useful marker for forensic semen identification. We further developed a TaqMan realtime PCR detection method to make it easier to analyze the methylation status of DACT1 region. This method can be a useful supplementary tool for semen identification, especially from aged stains.