The challenge to underlying causes of steroid insensitivity in bronchial asthma and its clinical application

我们研究了蛋白磷酸酶作为严重哮喘患者激素不敏感的潜在原因。我们重点研究了我们最近报道的磷酸酶以外的其他磷酸酶，并发现它们在重度哮喘患者的细胞中的蛋白表达水平降低。我们利用成像流式细胞仪优化了它们在每个细胞中的检测方法。此外，它们的表达水平与实际类固醇不敏感之间的关联正在研究中，这可能会导致未来的临床应用。

We examined the involvement of protein phosphatases as underlying causes of steroid insensitivity in severe asthma. We focused on phosphatases other than the phosphatase recently reported by us, and revealed that their protein expression levels were reduced in the cells obtained from severe asthmatics. We have optimized the detection methods of them in each cell by using imaging flow cytometer. In addition, the association between their expression levels and practical steroid insensitivity is under study, which might lead to a clinical application in future.